Command
CELLos · IRENA cell OS
Design a cell programme, translate it onto the Prepaire BSL-3 robot, and run coat–seed–feed–image–passage as one identity-safe loop. First acceptance: the seven-day WTC11 SAT.
Live deck
IRENA BSL-3 · Masdar
Dual gantry, plate nests and NeXCell incubator. SAT-7 · WTC11 feeder-free cycle
- Temp
- 37.04 °C
- CO₂
- 5.02%
- RH
- 94%
- Envelope
- BSL-3 released
Operator pack
Word technical documentation for a first shift, and the as-built PDF brochure of the Masdar workcell.
SAT-7 demonstration
IRENA-BIO-SOP-001 · day 3- D0Coat + seed
- D1Image and feed
- D2Trajectory
- D3Watch confluence
- D4Passage A / B
- D5Recovery
- D6Daily loop
- D7Export SAT
Differentiated area <5% green; 5–10% yellow; >10% red.
SAT-7 · WTC11 feeder-free cycle
IRENA-BIO-SOP-001 · day 3 · Image and feed
6-well · SBSIRENA-PLT-0147
Well A1GREEN
- Confluence
- 72.0%
- Diff. area
- 1.8%
- Viability
- 93.0%
- Residual iPSC
- 0.30%
Mean confluence · 74%
Interlocks
- Identity chainPlate IRENA-PLT-0147 matches incubator slot C3.OK
- Incubator37.04 °C · 5.02% CO₂ · 94% RH.OK
- OpticsInline imager not SAT. Classifier advisory only.GAP
- Waste / tipsWaste 34% · 1,280 filtered tips.OK
- BSL-3 envelopeRoom released. No contamination alert.OK
- Laser armDisarmed. Two-role authorisation required.OK
Sidecar jobs
Deck- Parr N₂ cavitationVES-0144running
- AO/PI cassette counterTUB-SAT7-Bready
- EasySep CD14TUB-CD14-03queued
- LSRFortessa-classPLT-V96-22queued
Queue
Current method
6.2b · Imager + pipette
Classifier confluence / morphology; feed
Green feed; yellow review
Event log
- 08:12:04Run SAT-7 released. WTC11 P18 identity matched.
- 08:14:21Vitronectin coating 58 min · no dry-well flag.
- 09:02:11Day 1 whole-well images exported. Mean confluence 28%.
- 09:58:40Day 2 feed complete. Exposure 6.4 min.
- 10:31:08Day 3 pre-feed image: 5/6 wells GREEN. B3 YELLOW (edge merge).
- 10:31:19B3 differentiated-area 6.1% — advisory hold on autonomous passage.